This research screens pansit-pansitan (Peperomia pellucida), an herb accepted by the populace as an effective cure for a variety of ailments which, unfortunately was proven to exhibit genetoxicity in the Allium Test. As part of the school’s herbal project, this research assessed the plant’s lethal activity using the Brine Shrimp Lethality Bioassay. The testing involved the exposure of Artemia salina nauplii to different aqueous extract concentrations (10 percent 30 percent, and 50 pecent) of the plant in three serial dilutions. Mortality among nauplii samples was observed for 48 hours in experimental and control groups in three replications, and LC50 value was computed using the Spearman-Karber Method. Significant differences among sample observations were determined by employing One-Way Analysis of Variance. Lethal activity of the test plant on the invertebrate model was proven with 9.18 LC50 value and 50 percent extract concentration as the most lethal treatment. This established bioactivity of pansit-pansitan.
One of the risks in herbal medicine is the lack of knowledge on the chemical properties of herbs. Because of this, the screening of plant metabolites has been integrated into many research. Some studies indicate that the local herbs pansitpansitan, sambong, and lagundi may possess levels of toxicity (Transactions of the NAST, Volume XIII, 1991). However, solid data on toxicity are yet to be established for the pansitpansitan. This research attempts a thorough evaluation and screening of the plant’s metabolites to ensure safety for human consumption. Since the properties of the test plant are not yet well documented, the findings of the study will be useful in establishing its bioactivities.
Materials & Equipment
Transactions of National Academy of Science and Technology, Volume XIX, 1998 Cantoria, Magdalena. 1998. Selected Topics in Pharmacognosy. National Academy of Science and Technology Publication.
Further clarification of the procedures and results should be directed to the researchers and adviser.
Chinkee I. Lim
Mrs. Christine Merca Obusan
Philippine Science High School
Bicol Region Campus