This research was conducted to determine the ability of “cacao” (Theobroma cacao) seed nicotinamide component to regulate angiogenesis or process of blood vessel formation in duck embryos. Abnormalities in angiogenesis are linked to the development of diseases such as cardiovascular ailments in case of insufficient blood vessel growth and cancer in case of excessive development. Varying “cacao” seed solutions were introduced into the chorion of duck embryos prior to the period of blood island formation.

After seven(7) days of incubation, the number, visibility, and extensiveness of blood vessels formed in the allantoic extra-embryonic circulatory system were observed for any angiogenic activity of the treatments. The number of main blood vessels observed in embryos treated with 2 g “cacao” solutions was significantly higher than that of the control group at 5% level of significance. On the other hand, the number of side branches of main blood vessels counted in embryos treated with 2g, 4g, and 6g “cacao” solution was significantly higher than that of the control group at 5%. It was found that 4g “cacao” solution showed the highest angiogenic property by promoting the most number and extensive branching of blood vessels. Compared with the control group, duck embryos treated with different “cacao” solutions significantly formed more blood vessels and promoted more extensive branching of blood vessel networks. Therefore Theobroma cacao seeds have angiogenic property and can be used to positively regulate blood vessel formation.

Materials & Equipment




Further clarification of the procedures and results should be directed to the researchers and adviser.

Researchers:
James Aaron D. Porlante
Ma. Cristina A. Valencia

Adviser:
Ms. Marie Christine W. Merca
Philippine Science High School-Bicol Region Campus